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December 22, 2020
What is PCR?
Polymerase chain reaction (PCR) is a method to amplify a target sequence from background nucleic acid . A way to make lots of copies of DNA.
Basic Components of PCR
• Target DNA (example: environmental DNA)
• 2 primers (20-30 nts long)
• dNTP’s
• Thermostable DNA polymerase
• Mg2+ (cofactor for DNA polymerase)
PCR laboratory, also known as gene amplification laboratory, uses molecular biology techniques to amplify DNA fragments, which can also be regarded as special DNA replication outside the organism. Using the gene tracking system to grasp the virus content in the human body, the precision of this test can reach the nanometer level.
The PCR laboratory does not have strict requirements on temperature. Considering the humanization of the laboratory, the human body feels more comfortable at ambient temperature (18℃~25℃, relative humidity: 50%). Therefore, the PCR laboratory should take various measures to ensure that the ambient temperature and humidity are controlled within a certain range.
At present, domestic secondary hospitals are basically actively carrying out gene amplification inspection laboratory projects, and the design and construction of PCR laboratories has attracted more and more attention from the medical community. According to the experimental process, the functions of the rooms are the sample preparation area, the sample preparation area, the gene amplification area, and the product analysis area. There is no requirement for cleanliness in this area. Purification cleanliness.
The PCR rapid detection workstation is a magic weapon to improve the detection time and save the cost of building a laboratory. So the concept of instant sampling and instant detection became popular.
The test result report will be issued within 30 minutes, from the machine to run the test after the sample is processed.