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How about building High Performance HPLC liquid chromatography analysis method for your laboratory?

May 10, 2016

The general steps of establishing a liquid chromatography analysis method include selecting a chromatography method according to the sample, selecting a chromatographic column, selecting a separation condition, qualitative and quantitative analysis, etc.


1. Select the chromatographic method:

Choose a suitable liquid chromatography method according to the characteristics of the sample.


2. Select the chromatographic column:

Select a suitable chromatographic column, determine the column size and stationary phase.


3. Select the separation conditions:

Select appropriate or optimized separation operating conditions, determine mobile phase composition, flow rate and elution method.


4. Qualitative and quantitative analysis:

Qualitative and quantitative analysis were carried out from the obtained chromatograms.


Bonnin team helps many testing institutions, chemical plants, pharmaceutical factories and universities to establish GC and LC chromatography laboratories!